Part:BBa_K1753001

RNA polymerase omega subunit

This part contains a sequence that codes for RNA polymerase omega subunit. Expressed with other 4 subunits, this subunit can be used to assemble RNA polymerase core enzyme.

It is the smallest subunit of bacterial RNA polymerase (amino acid 91) of E. coli. During assembly of a core enzyme it attaches last and contacts with b’-subunit and only with it. This subunit has been demonstrated to be essential for the correct folding of a b’-subunit, participate in attracting an s-subunit to a core enzyme and be a target for some transcriptional factors regulating the functioning of RNA polymerase (ppGpp).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Sequence:

ATGGCACGCGTAACTGTTCAGGACGCTGTAGAGAAAATTGGTAACCGTTTTGACCTGGTACTGGTCGCCGCGCGTCGCGCTCGTCAGATGCAGGTAGGCGGAAAGGATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAA